Ethanol Production Research Articles

The time-varying dynamic impact of US renewable energy prices on agricultural prices in China: the case of fuel ethanol.

This paper intends to look into the time-varying dynamic impact of US fuel ethanol, one of the renewable energy sources, on the prices of agricultural products (specifically corn, soybeans, rice, and wheat) in China based on monthly price data from January 2000 to January 2023. To achieve this, a time-varying parameter vector autoregressive (TVP-VAR) model is employed, which takes into account structural changes in emergencies through time-varying parameters. The empirical results show that the equal-interval impulse responses of price fluctuations in agricultural commodities are primarily positive to variations in fuel ethanol prices and production. And the intensity and direction of the effects vary at distinct time lags. Additionally, the magnitude of these responses is most pronounced in the short term for all agricultural commodities except for corn, and the duration of the impulse responses at different time points is generally longer for corn prices compared to other commodities. The study also reveals that the influence of US fuel ethanol on Chinese agricultural commodity prices is not substantial on the whole. Therefore, there is a necessity to advance the growth of biofuels and provide policy support and financial subsidies for agricultural products earmarked for food production. These actions could shed insights into the progression of Chinese renewable energy and food policies, ensuring the stability of the market in the long run.

Optimal trade-off between boosted tolerance and growth fitness during adaptive evolution of yeast to ethanol shocks

BackgroundThe selection of Saccharomyces cerevisiae strains with higher alcohol tolerance can potentially increase the industrial production of ethanol fuel. However, the design of selection protocols to obtain bioethanol yeasts with higher alcohol tolerance poses the challenge of improving industrial strains that are already robust to high ethanol levels. Furthermore, yeasts subjected to mutagenesis and selection, or laboratory evolution, often present adaptation trade-offs wherein higher stress tolerance is attained at the expense of growth and fermentation performance. Although these undesirable side effects are often associated with acute selection regimes, the utility of using harsh ethanol treatments to obtain robust ethanologenic yeasts still has not been fully investigated.ResultsWe conducted an adaptive laboratory evolution by challenging four populations (P1–P4) of the Brazilian bioethanol yeast, Saccharomyces cerevisiae PE-2_H4, through 68–82 cycles of 2-h ethanol shocks (19–30% v/v) and outgrowths. Colonies isolated from the final evolved populations (P1c–P4c) were subjected to whole-genome sequencing, revealing mutations in genes enriched for the cAMP/PKA and trehalose degradation pathways. Fitness analyses of the isolated clones P1c–P3c and reverse-engineered strains demonstrated that mutations were primarily selected for cell viability under ethanol stress, at the cost of decreased growth rates in cultures with or without ethanol. Under this selection regime for stress survival, the population P4 evolved a protective snowflake phenotype resulting from BUD3 disruption. Despite marked adaptation trade-offs, the combination of reverse-engineered mutations cyr1A1474T/usv1Δ conferred 5.46% higher fitness than the parental PE-2_H4 for propagation in 8% (v/v) ethanol, with only a 1.07% fitness cost in a culture medium without alcohol. The cyr1A1474T/usv1Δ strain and evolved P1c displayed robust fermentations of sugarcane molasses using cell recycling and sulfuric acid treatments, mimicking Brazilian bioethanol production.ConclusionsOur study combined genomic, mutational, and fitness analyses to understand the genetic underpinnings of yeast evolution to ethanol shocks. Although fitness analyses revealed that most evolved mutations impose a cost for cell propagation, combination of key mutations cyr1A1474T/usv1Δ endowed yeasts with higher tolerance for growth in the presence of ethanol. Moreover, alleles selected for acute stress survival comprising the P1c genotype conferred stress tolerance and optimal performance under conditions simulating the Brazilian industrial ethanol production.

Key role of K+ and Ca2+ in high-yield ethanol production by S. Cerevisiae from concentrated sugarcane molasses

BackgroundSaccharomyces cerevisiae is an important microorganism in ethanol synthesis, and with sugarcane molasses as the feedstock, ethanol is being synthesized sustainably to meet growing demands. However, high-concentration ethanol fermentation based on high-concentration sugarcane molasses—which is needed for reduced energy consumption of ethanol distillation at industrial scale—is yet to be achieved.ResultsIn the present study, to identify the main limiting factors of this process, adaptive laboratory evolution and high-throughput screening (Py-Fe3+) based on ARTP (atmospheric and room-temperature plasma) mutagenesis were applied. We identified high osmotic pressure, high temperature, high alcohol levels, and high concentrations of K+, Ca2+, K+ and Ca2+ (K+&Ca2+), and sugarcane molasses as the main limiting factors. The robust S. cerevisiae strains of NGT-F1, NGW-F1, NGC-F1, NGK+, NGCa2+ NGK+&Ca2+-F1, and NGTM-F1 exhibited high tolerance to the respective limiting factor and exhibited increased yield. Subsequently, ethanol synthesis, cell morphology, comparative genomics, and gene ontology (GO) enrichment analysis were performed in a molasses broth containing 250 g/L total fermentable sugars (TFS). Additionally, S. cerevisiae NGTM-F1 was used with 250 g/L (TFS) sugarcane molasses to synthesize ethanol in a 5-L fermenter, giving a yield of 111.65 g/L, the conversion of sugar to alcohol reached 95.53%. It is the highest level of physical mutagenesis yield at present.ConclusionOur results showed that K+ and Ca2+ ions primarily limited the efficient production of ethanol. Then, subsequent comparative transcriptomic GO and pathway analyses showed that the co-presence of K+ and Ca2+ exerted the most prominent limitation on efficient ethanol production. The results of this study might prove useful by promoting the development and utilization of green fuel bio-manufactured from molasses.

Comprehensive Evaluation of Fuel Ethanol Production from Poplar Wood Fermentation and its Coupled Pathway with Lignin Gasification Based on Energy-Environment-Economy

Comprehensive Evaluation of Fuel Ethanol Production from Poplar Wood Fermentation and its Coupled Pathway with Lignin Gasification Based on Energy-Environment-Economy

Rapid fractionation of corn stover by microwave-assisted protic ionic liquid [TEA][HSO4] for fermentative acetone–butanol–ethanol production

BackgroundThe use of ionic liquids (ILs) to fractionate lignocelluloses for various bio-based chemicals productions is in the ascendant. On this basis, the protic ILs consisting of triethylammonium hydrogen sulfate ([TEA][HSO4]) possessed great promise due to the low price, low pollution, and high efficiency. In this study, the microwave-assistant [TEA][HSO4] fractionation process was established for corn stover fractionation, so as to facilitate the monomeric sugars production and supported the downstream acetone–butanol–ethanol (ABE) fermentation.ResultsThe assistance of microwave irradiation could obviously shorten the fractionation period of corn stover. Under the optimized condition (190 W for 3 min), high xylan removal (93.17 ± 0.63%) and delignification rate (72.90 ± 0.81%) were realized. The mechanisms for the promotion effect of the microwave to the protic ILs fractionation process were ascribed to the synergistic effect of the IL and microwaves to the depolymerization of lignocellulose through the ionic conduction, which can be clarified by the characterization of the pulps and the isolated lignin specimens. Downstream valorization of the fractionated pulps into ABE productions was also investigated. The [TEA][HSO4] free corn stover hydrolysate was capable of producing 12.58 g L−1 of ABE from overall 38.20 g L−1 of monomeric sugars without detoxification and additional nutrients supplementation.ConclusionsThe assistance of microwave irradiation could significantly promote the corn stover fractionation by [TEA][HSO4]. Mass balance indicated that 8.1 g of ABE and 16.61 g of technical lignin can be generated from 100 g of raw corn stover based on the novel fractionation strategy.

Evaluation of commercial importance of endophytes isolated from Argemone mexicana and Papaver rhoeas.

The paper industry is a composite one constituting different types of mills, processes, and products. The paper industries consume large amounts of resources, like wood and water. These industries also create huge amounts of waste that have to be treated. In our study, 23 endophytic bacteria were isolated from Argemone mexicana, and 16 endophytic bacteria were isolated from Papaver rhoeas. Seventeen and 15 bacterial endophytes from A. mexicana and P. rhoeas, respectively, showed cellulose-degrading activity. The biochemical and molecular characterization were done for endophytic bacteria with cellulolytic activity. The consortium of cellulose-degrading endophytic bacteria from A. mexicana showed endoglucanase activity (0.462IU/ml) and FPCase enzyme activity (0.269IU/ml) and from P. rhoeas gave endoglucanase activity (0.439IU/ml) and FPCase enzyme activity (0.253IU/ml). Degraded carboxy methylcellulose and filter paper were further treated by Saccharomyces cerevisiae and bioethanol was produced. Cellulose-degrading endophytic bacteria were also tested for auxin, siderophore production, and phosphate solubilization activities. Individual cellulose-degrading endophytic bacteria with plant growth-promoting activities were used as biofertilizers, tested for plant growth-promoting activities using Basmati Pusa 1121 rice, and plant growth parameters were recorded. The degraded paper enhances the growth of rice plants. Selected bacterial endophytes and their consortia from A. mexicana and P. rhoeas were powerful cellulose degraders, which can be further employed for ethanol production and as significant biofertilizers in agriculture.

Boron Impact on Maize Growth and Yield: A Review

Maize (Zea mays L.) is a vital crop, contributing significantly—at least 30%—to global dietary energy intake and biofuels and ethanol production. This review article delves into the dynamic interplay between boron (B) and maize growth, yield, and agricultural sustainability. Boron, a crucial micronutrient, is pivotal in essential physiological processes such as root development, leaf expansion, and cob formation. These processes are fundamental for ensuring the vigour and productivity of maize crops. Conversely, boron deficiency manifests as thinner leaves with reduced chlorophyll content, compromising plant health, and hindering yield potential. Maintaining adequate boron levels, particularly during reproductive stages, is critical for mitigating the risk of abnormal ears and maximizing the quantity and quality of maize production. Emerging research underscores the significance of foliar boron application at various growth stages of maize, which stimulates growth, facilitates cell wall development, and increases leaf area. This translates to improved light interception and photosynthetic efficiency, ultimately contributing to increased plant vigour and biomass accumulation. Furthermore, exploring innovative approaches for sustainable boron management is crucial. This includes precision fertilization techniques and biofortification strategies to ensure optimal maize production while minimizing environmental impacts. By gaining a deeper understanding of the complex relationship between boron and maize, farmers can develop customized fertilization plans that utilize strategic foliar boron application. This approach unlocks maize's full yield potential and contributes to sustainable agricultural practices, supporting global food security.

Enhancing complex bioprocess learning through simulation technology and hybrid teaching: A case study in university education.

The utilization of computer simulators in university education is progressively being embraced to offer students a practical exposure to industrial bioprocesses. Bioreactor computer simulators hold various advantages over conventional laboratory experiments, such as cost-effectiveness and enhanced safety. The research objective is to assess the effectiveness of integrating bioreactor computer simulators into hybrid teaching to promote active and collaborative learning experiences and evaluate their impact on student participation and understanding. A hybrid strategy combining synchronous, face-to-face, and online teaching has been implemented to enhance the teaching-learning processes in the Industrial Bioprocesses course for Biochemistry students. The simulation software BIOSTAT®T Yeast was used. This software models the production of ethanol with Saccharomyces cerevisiae through batch cultivation and the determination of the kLa value of a bioreactor. In the first simulation activity, students analyzed the software response based on parameter values input by the instructor, while in the second simulation activity, students autonomously used the computer simulator under the primary oversight of the instructor. The survey results indicate that the pedagogical innovation was positively received and significantly motivating for the students. Comparing student satisfaction surveys between the two simulation activities suggests that fostering student autonomy and engagement through simulation technology can improve satisfaction and learning outcomes in bioprocess education.

Bottom‐up Growth of Convex Sphere with Adjustable Cu(0)/Cu(I) Interfaces for Effective C2 Production from CO2 Electroreduction

One challenge confronting the Cu2O catalysts in the electrocatalysis of carbon dioxide reduction reaction (CO2RR) is the reduction of active Cu(I) species, resulting in low selectivity and quick deactivation. In this study, we for the first time introduce a bottom‐up growth of convex sphere with adjustable Cu(0)/Cu(I) interfaces (Cux@Cu2O convex spheres). Interestingly, the interfaces are dynamically modulated by varying hydrothermal time, thus regulating the conversion of C1 and C2 products. In particular, the 4 h hydrothermal treatment applied to Cu0.25@Cu2O convex sphere with the favorable Cu(0)/Cu(I) interface results in the highest selectivity for C2 products (90.5%). In situ Fourier‐transform infrared spectroscopy measurements and density functional theory calculations reveal that the Cu(0)/Cu(I) interface lowers the energy barrier for the production of ethylene and ethanol while increasing the coverage of localized *CO adsorbate for increased dimerization. This work establishes a novel approach for transforming the state of valence‐sensitive electrocatalysts into high‐value energy‐related engineering products.

Bottom-up Growth of Convex Sphere with Adjustable Cu(0)/Cu(I) Interfaces for Effective C2 Production from CO2 Electroreduction.

One challenge confronting the Cu2O catalysts in the electrocatalysis of carbon dioxide reduction reaction (CO2RR) is the reduction of active Cu(I) species, resulting in low selectivity and quick deactivation. In this study, we for the first time introduce a bottom-up growth of convex sphere with adjustable Cu(0)/Cu(I) interfaces (Cux@Cu2O convex spheres). Interestingly, the interfaces are dynamically modulated by varying hydrothermal time, thus regulating the conversion of C1 and C2 products. In particular, the 4 h hydrothermal treatment applied to Cu0.25@Cu2O convex sphere with the favorable Cu(0)/Cu(I) interface results in the highest selectivity for C2 products (90.5%). In situ Fourier-transform infrared spectroscopy measurements and density functional theory calculations reveal that the Cu(0)/Cu(I) interface lowers the energy barrier for the production of ethylene and ethanol while increasing the coverage of localized *CO adsorbate for increased dimerization. This work establishes a novel approach for transforming the state of valence-sensitive electrocatalysts into high-value energy-related engineering products.

163 Impact of feeding wet or dry traditional or fractionated (Solbran) distiller’s grains on feedlot cattle performance and carcass characteristics

Abstract Solbran (ICM, Colwich, KS) is new corn byproduct of ethanol production composed of fiber plus condensed distiller’s solubles and can be fed dry (Dry Solbran; NDF = 41.1%; CP = 21.84%; Ether extract = 8.4%), or wet (Wet Solbran; NDF = 49.7%; CP = 22.67%; Ether extract = 8.5%). The objective of this study was to evaluate the energy value of dry and wet Solbran compared with traditional dry- (DDGS) and wet- distiller’s grains plus solubles (WGGS) in beef cattle finishing diets. The treatments included a control (CON) with no distiller’s grains compared with the inclusion of DDGS, WDGS, Dry Solbran and Wet Solbran at two levels (20% and 40%) resulting in a 2 × 2 × 2 + 1 factorial. Orthogonal contrasts defined linear and quadratic effects of byproduct inclusion through the common intercept of CON. Additionally, interactions among inclusion (20% or 40%), moisture (dry and wet) and processing method (Solbran or traditional) were evaluated as a 2 × 2 × 2 factorial without CON. There was a linear increase in dry matter intake (DMI) by adding DDGS, Dry Solbran, and Wet Solbran (P < 0.01) and a tendency for a linear increase in dry matter intake (DMI) with WDGS (P = 0.07). There was a linear increase in ADG by adding DDGS or Wet Solbran (P < 0.05) and a tendency for a linear increase in average daily gain (ADG) with the addition of Dry Solbran or WDGS (P < 0.10). These changes in DMI and ADG resulted in a linear decrease in gain to feed ratio (G:F) when Dry Solbran was added (P < 0.01), and a tendency towards a linear decrease in G:F when DDGS was added (P < 0.10). The inclusion of Wet Solbran or WDGS did not influence G:F (P = 0.45). For the 2 × 2 × 2 factorial, there was a 2-way interaction for moisture by inclusion of byproduct for G:F where wet byproduct fed at 40% of the diet resulted in the greatest G:F and 40% dry byproduct resulted in the least G:F (P = 0.04). This interaction was driven by an increase in DMI for 40% byproduct when fed dry, and a reduction in DMI for both 20 and 40% byproduct fed wet. There was no difference in ADG between the treatments (P = 0.75). For the main effect byproduct type, steers fed Solbran had greater DMI (P < 0.01) with no difference in ADG (P = 0.79), resulting in a 3% reduction in G:F (P = 0.03) for steers fed Solbran but greater marbling (P < 0.01). Impact of feeding distillers grains byproducts on DMI, ADG, and G:F are impacted by the processes used in the ethanol plant, whether wet or dry, and dietary inclusion.

Taurine-mediated gene transcription and cell membrane permeability reinforced co-production of bioethanol and Monascus azaphilone pigments for a newly isolated Monascus purpureus

BackgroundTaurine, a semi-essential micronutrient, could be utilized as a sulfur source for some bacteria; however, little is known about its effect on the accumulation of fermentation products. Here, it investigated the effect of taurine on co-production of bioethanol and Monascus azaphilone pigments (MonAzPs) for a fungus.ResultsA newly isolated fungus of 98.92% identity with Monascus purpureus co-produced 23.43 g/L bioethanol and 66.12, 78.01 and 62.37 U/mL red, yellow and orange MonAzPs for 3 d in synthetic medium (SM). Taurine enhanced bioethanol titer, ethanol productivity and ethanol yield at the maximum by 1.56, 1.58 and 1.60 times than those of the control in corn stover hydrolysates (CSH), and red, yellow and orange MonAzPs were raised by 1.24, 1.26 and 1.29 times, respectively. Taurine was consumed extremely small quantities for M. purpureus and its promotional effect was not universal for the other two biorefinery fermenting strains. Taurine intensified the gene transcription of glycolysis (glucokinase, phosphoglycerate mutase, enolase and alcohol dehydrogenase) and MonAzPs biosynthesis (serine hydrolases, C-11-ketoreductase, FAD-dependent monooxygenase, 4-O-acyltransferase, deacetylase, NAD(P)H-dependent oxidoredutase, FAD-dependent oxidoredutase, enoyl reductase and fatty acid synthase) through de novo RNA-Seq assays. Furthermore, taurine improved cell membrane permeability through changing cell membrane structure by microscopic imaging assays.ConclusionsTaurine reinforced co-production of bioethanol and MonAzPs by increasing gene transcription level and cell membrane permeability for M. purpureus. This work would offer an innovative, efficient and taurine-based co-production system for mass accumulation of the value-added biofuels and biochemicals from lignocellulosic biomass.

A Density Functional Theory Analysis of Electrochemical Oxidation of Methane to Alcohol over High-Entropy Oxide (CoCrFeMnNi)3O4 Catalysts.

The direct conversion of methane into alcohol is a promising approach for achieving a low-carbon future, yet it remains a major challenge. In this study, we utilize density functional theory to explore the potential of the (CoCrFeMnNi)3O4 (CCFMNO) high entropy oxide (HEO) for electrochemical oxidation of methane to methanol and ethanol, alongside their competition with CO2 production. Our primary focus in this study is on thermodynamics, enabling a prompt analysis of the catalyst's potential, with the calculation of electrochemical barriers falling beyond our scope. Among all potential active sites within CCFMNO HEO, we identify Co as the most active site for methane activation when using carbonate ions as oxidants. This results in methanol production with a limiting potential of 1.4 VCHE, and ethanol and CO2 productions with a limiting potential of 1.2 VCHE. Additionally, our findings suggest that the occupied p-band center of O* on CCFMNO HEO is a potential descriptor for identifying the most active site within CCFMNO HEO. Overall, our results indicate that CCFMNO HEO holds promise as catalysts for methane oxidation to alcohols, employing carbonate ions as oxidants.

A catalog of ethanol-producing microbes in humans.

Aim: Endogenous ethanol production emerges as a mechanism of nonalcoholic steatohepatitis, obesity, diabetes and auto-brewery syndrome. Methods: To identify ethanol-producing microbes in humans, we used the NCBI taxonomy browser and the PubMed database with an automatic query and manual verification. Results: 85 ethanol-producing microbes in human were identified. Saccharomyces cerevisiae, Candida and Pichia were the most represented fungi. Enterobacteriaceae was the most represented bacterial family with mainly Escherichia coli and Klebsiellapneumoniae. Species of the Lachnospiraceae and Clostridiaceae family, of the Lactobacillales order and of the Bifidobacterium genus were also identified. Conclusion: This catalog will help the study of ethanol-producing microbes in human in the pathophysiology, diagnosis, prevention and management of human diseases associated with endogenous ethanol production.

Cofactor engineering in Thermoanaerobacterium aotearoense SCUT27 for maximizing ethanol yield and revealing an enzyme complex with high ferredoxin-NAD+ reductase activity

Thermoanaerobacterium aotearoense SCUT27 is a prominent producer of biofuels from lignocellulosic materials. To provide sufficient NAD(P)H for ethanol production, redox-related genes, including lactate dehydrogenase (ldh), redox-sensing transcriptional repressor (rex), and hydrogenase (hfsB), were knocked out. However, the growth of strain PRH (Δldh/Δrex/ΔhfsB) was suppressed due to the intracellular redox state imbalance with the increased NADH concentration. Coincidentally, when the Bcd–EtfAB (BCD) complex was overexpressed, the resulting strain PRH–B3 (Δldh/Δrex/ΔhfsB::BCD) grew rapidly and produced ethanol with a high yield. With lignocellulosic hydrolysates, PRH-BA (Δldh/Δrex/ΔhfsB::BCD::adhE) demonstrated high ethanol productivity and yield, reaching levels of 0.45–0.51 g/L/h and 0.46–0.53 g/g sugars, respectively. The study results shed light on the cofactor balance for cell stability and the high ferredoxin–NAD+ reductase activity of the BCD complex under an intracellular low redox state. They also provide an essential reference for developing strains for improved biofuel production.

Comparison of lactic and propionic acid hydrolysis for production of xylo-oligosaccharides and ethanol from polysaccharides in Toona sinensis branch

Xylan-type hemicellulose hydrolysis by an organic acid solution for the production of xylo-oligosaccharides (XOS) is efficient and eco-friendly, but the effects of different organic acids on XOS production from Toona sinensis branch (TB) biomass is limited. In this work, under the conditions of 170 °C for 60 min, 33.1 % and 38.7 % XOS yields were obtained from polysaccharides present in TB by 2 % lactic acid (LA) and 6 % propionic acid (PA), respectively. Then 77 % of the lignin was removed by hydrogen peroxide−acetic acid pretreatment system, and 39.5 % and 44.7 % XOS yield were obtained from polysaccharides in delignification TB by 2 % LA and 6 % PA, respectively. It was found that PA hydrolysis, especially from delignified TB, resulted in higher XOS yield and purity compared to LA hydrolysis. Moreover, the content of byproducts (xylose, hydroxymethyl-furfural and furfural) in PA hydrolysate was lower. Following the hydrolysis process, the simultaneous saccharification and fermentation of the TB solid residue achieved an ethanol yield of 71.5 %. This work proposes an integrated process to preferentially converts the TB hemicellulose into valuable XOS and then converts the cellulose into ethanol. This process had the advantage of eliminating the need for isolation and purification, and the potential to obtain multiple products from the same raw material.

Effect of water content in ethanol on flame front wrinkling and curvature in a spark-ignition engine using OH PLIF imaging

The production of ethanol fuel involves separating water and ethanol by distillation and dehydration in an energy intensive process. To reduce the carbon footprint, hydrous ethanol is seen as a promising alternative. This study focused on the effect of water content in ethanol on flame development in a single-cylinder optical spark-ignition engine. Ethanol with 0%, 4% and 10% water content per volume was used with Port Fuel Injection (PFI) and Direct Injection (DI) for homogeneous and heterogeneous mixture preparation. Tests were also conducted with iso-octane for comparison. Flames captured by Planar Laser Induced Fluorescence (PLIF) of OH were spatially filtered to remove scales larger than the flame size, then the flame brush was quantified in terms of thickness, crossing frequency between instantaneous and filtered contours, and curvature. Increasing water content resulted in slower flame growth, with 10% hydrous exhibiting marginally higher flame speed than iso-octane. PFI led to faster flame growth and thicker flame brushes than DI at the same timing after ignition. When the brush thickness was normalised by flame radius, all hydrous fuels and iso-octane exhibited similar values with PFI, whilst anhydrous ethanol exhibited the thickest normalised brush. The alcohols maintained similar normalised thicknesses with DI and iso-octane exhibited the thickest normalised brush. When flames of the same radii were considered with PFI at the same timing, the fuels with sequentially lower laminar burning velocity were associated with larger crossing frequency. For PFI, increasing water content resulted in decreased flame roundness irrespective of size, with the 4% hydrous and iso-octane exhibiting similar values. With DI, anhydrous ethanol flames were rounder than the 4% hydrous when averaged over all cycles, but marginally rounder than the 10% hydrous and iso-octane. Increasing water content widened the curvature distribution and increased the mean with PFI. Flame roundness decreased with increase in mean curvature and Lewis number for both PFI and DI.

Enumeration, Identification and characterization of endophytic bacteria isolated from sugar beet (Beta vulgaris L.) root for ethanol production

The bioethanol production from sugar beet is regulated by endophytic bacteria. Thus, the present investigation is aimed to isolate, identify and characterize endophytic bacteria from different varieties of sugar beet roots. The presence of specific bacteria in sugar beet roots plays vital role in bioethanol production. The ability to produce bioethanol through fermentation with the help of various microbes present in roots was studied in different varieties of sugar beet at Indian Institute of Sugarcane Research, Lucknow sub tropical regions of India. A total of 16 microbial isolates comprising of 12 bacterial and 4 fungal strains were studied for their potential to produce bioethanol from sugar beet root. Among them only 2 bacteria were more effective as fermenting agents. These isolated endophytic bacteria were characterised by molecular sequencing and were identified as Enterobacter hormaechei and Enterobacter cloacae. These isolates may also act as a good alternative agent for the bioethanol production.

Unlocking waste potential: A neural network approach to forecasting sustainable acetaldehyde production from ethanol upcycling in biomass waste gasification

The upcycling of biomass waste gasification process for ethanol and acetaldehyde production has been augmented by integrating secondary and tertiary processes. A simulation model using Aspen Plus, developed from experimental investigations to assess the viability of this proposed system. Furthermore, this model has been utilized to create an Artificial Neural Network (ANN) prediction model. Process sustainability analysis demonstrated an energy efficiency of 64 % while economic viability up to 80 % process efficiency with an Internal Rate of Return (IRR) of 6 % and a payback period of 2107 days. An optimization strategy and an artificial neural network (ANN)-based predictive model have been developed. Optimization results revealed that a gasifier temperature of around 600 °C, a gasifying agent ratio of 2.0, and an acetaldehyde reactor temperature of 300 °C yield better process outcomes and revenue. The ANN model exhibited robust performance with coefficient of determination (R2) values ranging from 0.92 to 0.98 for acetaldehyde, hydrogen, and total revenue. Mean absolute error (MAE) and mean absolute percentage error (MAPE) fell within the range of 0.03–0.1 and 0.1–1.12 %, respectively. Consequently, given the favorable sustainability and predictive model performance, this study can be used for similar endeavors.

Co-fermentation exploiting glucose and xylose utilizing thermotolerant S. cerevisiae of highly lignified biomass for biofuel production: Statistical optimization and kinetic models

The present study assesses the potential of co-fermentation for the enhanced production of biofuels from a highly lignified biomass, Viz., Pomegranate Peel (PP) and Mosambi Peel (PP). The simultaneous saccharification and co-fermentation (SScF) on brewer's yeast Saccharomyces cerevisiae and modified strain S. cerevisiae XUSE were used for the ethanol production using RSM. The Box-Behnken design (BBD) model was used to execute 15 runs with independent factors of initial pH (5.5, 6.5, 7.5), yeast concentration (4%, 5% and 6%v/v), and co-culture yeast concentration (4%, 5% and 6%v/v). The highest bioethanol production was achieved by PP (35.460 ± 0.32 g/L) and MP (25.630 ± 0.40 g/L) on optimal variables of pH 6.5, with yeast concentration (5% v/v) and co-culture yeast concentration (5% v/v) for 120 hrs duration. Furthermore, the kinetic of logistic and modified Gompertz model analyzed the lag phase of PP and MP was 7.9 and 7.15 h, for which maximum bioethanol production (EtOH max-Pm) reported for PP (38.62 g/L) and MP (29.21 g/L), respectively. The study infers that SScF has great potential for producing high-titer ethanol commercially and supports waste-to-energy strategies.